The principles of spindle bipolarity
نویسندگان
چکیده
منابع مشابه
Aurora A, mitotic entry, and spindle bipolarity.
The kinase Aurora-A (Aur-A), which is enriched at centrosomes, is required for centrosome maturation and accurate chromosome segregation, and recent work implicates centrosomes as sites where the earliest activation of cyclin B1-cdc2 occurs. Here, we have used Xenopus egg extracts to investigate Aur-A's contribution to cell cycle progression and spindle morphology in the presence or absence of ...
متن کاملPolycystic kidney disease protein fibrocystin localizes to the mitotic spindle and regulates spindle bipolarity.
Autosomal recessive polycystic kidney disease (ARPKD) is a significant hereditary renal disease occurring in infancy and childhood, which presents with greatly enlarged echogenic kidneys, ultimately leading to renal insufficiency and end-stage renal disease. ARPKD is caused by mutations in a single gene PKHD1, which encodes fibrocystin/polyductin (FPC), a large single transmembrane protein gene...
متن کاملAurora A phosphorylates MCAK to control ran-dependent spindle bipolarity.
During mitosis, mitotic centromere-associated kinesin (MCAK) localizes to chromatin/kinetochores, a cytoplasmic pool, and spindle poles. Its localization and activity in the chromatin region are regulated by Aurora B kinase; however, how the cytoplasmic- and pole-localized MCAK are regulated is currently not clear. In this study, we used Xenopus egg extracts to form spindles in the absence of c...
متن کاملThe Role of Hklp2 in the Stabilization and Maintenance of Spindle Bipolarity
Spindle bipolarity relies on a fine balance of forces exerted by various molecular motors [1-4]. In most animal cells, spindle bipolarity requires sustained outward forces to push the spindle poles apart, an activity that is provided by Eg5, a conserved homotetrameric plus-end-directed kinesin that crosslinks and slides antiparallel microtubules apart [5]. These pushing forces are balanced by i...
متن کاملA model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity
BACKGROUND In eukaryotes, assembly of the mitotic spindle requires the interaction of chromosomes with microtubules. During this process, several motor proteins that move along microtubules promote formation of a bipolar microtubule array, but the precise mechanism is unclear. In order to examine the roles of different motor proteins in building a bipolar spindle, we have used a simplified syst...
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ژورنال
عنوان ژورنال: Nature Reviews Molecular Cell Biology
سال: 2019
ISSN: 1471-0072,1471-0080
DOI: 10.1038/s41580-019-0135-1